Dose dependent effects of acetaminophen and ibuprofen on mitochondrial respiration of human platelets Molecular and Cellular Biochemistry Springer Nature Link Is Acetaminophen or Ibuprofen better for kids? THE PEDIATRICIAN MOM Current Insights into Glutathione Depletion in Adult Septic Patients Ibuprofen administration attenuates serum TNF levels, hepatic glutathione depletion, hepatic apoptosis and mouse mortality after Fas stimulation ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.