Acetaminophen Glutathione d3 Disodium Salt (~90%) Avoid Tylenol with vaccines. A key reminder often emphasized in vaccine education is to avoid using Tylenol (acetaminophen) alongside vaccination. Tylenol requires glutathione for proper metabolism in the body. When glutathione As a naturopathic doctor and a mom, Tylenol is a hard no in my house and I want to explain why. , , Acetaminophen (Tylenol) is processed through the liver, and in that process it can lower Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.