Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Did you know that drugs like Tylenol and ibuprofen deplete the body's chief detoxifier, GLUTATHIONE? This is a BIG DEAL. Glutathione is essential not only for detox but also IMMUNE function, Early life acetaminophen exposure, glutathione S transferase genes, and development of adolescent asthma in a high risk birth cohort Journal of Allergy and Clinical Immunology The Truth About Paracetamol and Glutathione Levels What To Avoid When Taking Glutathione